Semen is a whitish or greyish liquid, but can occasionally appear yellowish,

emitted from the urethra (tube in the penis) on ejaculation.

Normal Sperm Count Morphology Motility 20 to 150 million/ml

Sperm count below 20 million/ml called Oligospermia.

When a Sexologist (Sex Doctor),advices Semen Analysis of a patient then the patient is require to give the sample for this.

  • The sample should be collected after 3-4 days of sexual abstinence.
  • Typically, semen collection will require masturbation and collection of the semen into a sterile container.
  • Anyone who is having trouble conceiving should have a semen analysis done. In about 40% of infertile couples, the cause of the infertility lies with the males, and this could easily and quickly be diagnosed via a semen analysis.

The various parameters tested are:

  • Semen volume
  • Sperm concentration
  • Sperm count
  • Percentage motile sperm
  • Grade of motility
  • Normal sperm morphology

Semen consists  of the secretions of several glands but only 5 per cent comes from the testicles, the remaining part comes in this way:-

46 to 80 per cent  of semen comes from Seminal vesicles, 13 to 33 per cent comes from Prostate gland , glands2 to 5 per cent comes from the Bulbourethral and urethral.

Contents of semen are like this

ascorbic acid (vitamin C, for tissue maintenance)
blood-group antigens (from immune system)
calcium (mineral)
chlorine (oxidizing agent)
cholesterol (steroid alcohol present in body fluids)
chlorine (base, part of the vitamin B complex)
citric acid (occurs during cellular metabolism)
creatine (nitrogenous substance found in muscle)
deoxyribonucleic acid (DNA)
fructose (sugar used for energy)
glutathione (peptide amino acid)
Glycoproteins (cancer fighting agent)
hyaluronidase (enzyme)
inositol (sugar found in muscles)
lactic acid (byproduct of muscle use)
magnesium (mineral)
nitrogen (gas found in all living tissue)
phosphorus (mineral)
prostaglandins (good for pregnancy)
potassium (mineral)
purine (compound of uric acid)
pyrimidine (organic base)
pyruvic acid (formed from either glucose or glycogen)
selenium (cancer fighting agent)
sodium (salt)
sorbitol (body alcohol)
spermidine (catalytic enzyme)
spermine (ammonia compound found in sperm)
urea (from urine)
uric acid (from urine)
vitamin B12 (for proper function of nervous system and metabolism)
zinc (mineral)

Normal Semen Characteristics

Volume (ml) > or = 2
pH 7.2-8.0
Sperm concentration (M/ml) > or = 20
Total sperm count (M/ejaculate) > or = 40
Morphology (% normal) > or = 30
Vitality (% live) > or = 75
WBC (M/ml) <1.0
Immunobead test (%sperm with beads) < or = 10 <20
MAR test (%sperm with RBCs) <10
Calorie equivalent in ejaculate 9 calories
Motility Within 1h of ejaculation
class a (%) > or = 25
classes a and b (%) > or = 50
Neutral alpha-glucosidase (mU/ejaculate) > or = 20
Total zinc (µmol/ejaculate) > or = 2.4
Total citric acid (µmol/ejaculate) > or = 52
Total acid phosphates (U/ejaculate) > or = 200
Total Fructose (µmol/ejaculate) > or = 13


Dr. Satinder Singh

Phone: +91-94165 34999, +91-94670 15252


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